The unique and innovative XNAPS plasmid flexspin purification system provides a highly flexible, fast, simple, and efficient method for isolating plasmid DNA from mini (1 ml) to midi (150 ml) volumes of bacterial culture. The system combines the rapidity and simplicity of the spin column method with the flexibility of batch processing for plasmid DNA purification. The novel procedure provided by the kit eliminates many disadvantages, such as time-consuming methods, toxic components, low yield, as compared to other commercial kits.
The purified plasmid DNA is immediately ready to use in various downstream applications, such as PCR, restriction enzyme digestion, labeling, sequencing, transformation, regular transfection, and in vitro transcription/translation.
1. Rapidity: Depending on the culture volume, all the processing steps can be completed within 8-20 minutes.
2. High flexibility: The kit can be used efficiently from small culture volume (1-10 ml) to midi volume (150ml), even to maxi volume.
3. High yield and purity: Up to 30-500 ¦Ìg of high-copy plasmid DNA with a ratio of OD260/OD280 between 1.8-2.1can be extracted from 3 ml-150ml bacterial culture.
4. Safety for handling, shipping and storage: No phenol extraction, no ethanol precipitation, no toxic chaotropic salts.