XNAPS Blood gDNA Flexspin Kit

XNAPS Blood gDNA Flexspin Kit
Item# P1014A
Regular price: $80.00
Sale price: $75.00

Product Description

XNAPS blood gDNA flexspin kit provides a highly flexible, fast, and efficient method for isolating genomic DNA from up to 10ml of whole blood or equivalent buffy coat. The system combines a modified enzymatic lysis procedure with an innovative chromatographic absorbent resin which preferentially binds DNA with high capacity . Removal of red blood cells and plasma firstly not only enables maximum elimination of enzymatic inhibitors, but also increase dramatically the yield of purified DNA. The released free-form DNA after WBC lysis binds specifically with modified resin in aqueous circumstance. After the impurities are washed away and spun off, The binding DNA are eluted by pure water. The novel procedure provided by the kit eliminates many disadvantages, such as time-consuming methods, toxic components, low yield, as compared to other commercial kits. The purified genomic DNA is immediately ready to use in various downstream applications, such as PCR, restriction enzyme digestion, sequencing.

Features: 1. Flexibility: Extract efficiently genomic DNA from 10 Ʀ to 10ml of whole blood.

2. Rapidity: All the processing steps can be completed within 20 minutes.

3. High yield and purity: Up to 150g of genomic DNA with a ratio of OD260/OD280 between 1.8-2.0 can be extracted from 3ml of fresh whole blood.

4. Safety for handling, shipping and storage: No phenol extraction, no ethanol precipitation, no toxic chaotropic salt are necessary.

5. The molecular weight of purified genomic DNA is 30-50 kb, suitable for downstream manipulations.