UniversalTM One-step IHC is a latest breakthrough technology (patent pending) for performing Immunohistochemistry (IHC) or Immunocytochemistry (ICC). Unlike regular (classical) IHC/ICC, the One-step IHC/ICC technology combines blocking, primary antibody staining, washing and secondary antibody staining into a rapid one-step reaction. Therefore, using UniversalTM One-step IHC kit, your IHC/ICC becomes so simple and is no longer a time-consuming and labor-intensive procedure. The One-step IHC kit is a universal kit, which is suitable for most primary antibodies and no need of secondary antibody. Currently, we provide six types of UniversalTM One-step IHC kits as shown on the above table. Each kit is sufficient to stain 200 slides.
Immunohistochemistry (IHC) or Immunocytochemistry (ICC).
Easy, a simple One-step reaction instead of the 4-step procedure: blocking, primary antibody-binding, washing and 2nd antibody-binding.
Rapid, just 1~2 hrs instead of 4~5 hrs.
Universal, suitable for most primary antibodies and no need of secondary antibody.
Sensitive, comparable sensitivity with regular IHC or ICC.
Reproducible, highly reproducible results.
• Pretreatment Solution and Enhancer: for continuous use, store in 2~8 íŠ for up to 1 month, or store in -20 íŠ for up to a year.
• IHC Probe and Substrate: for continuous use, store in -20 íŠ for up to 6 months or store in -70 íŠ manual defrost freezer for up to a year.
Repeated freezing and thawing is not recommended.
• Rapid Wash Solution: store in room temperature for up to one year.
Note: Kit will be shipped in ambient temperature.
Comparison of One-step IHC with Regular IHC: