UniversalTM One-step Cell-based ELISA is a latest breakthrough technology (patent pending) for directly detectingprotein expression inside cells. Unlike regular (classical) ELISA, the One-step Cell-based ELISA is not only no needs of making cell lysat and coating plate but also replaces blocking, primary antibody binding, washing and secondary antibody binding with a rapid one-step reaction. Therefore, using UniversalTM One-step Cell-based ELISA kit, you can easily get your protein expression results just around one hour. The One-step Cell-based ELISA kit is a universal kit, which is suitable for most primary antibodies and no need of secondary antibody. Currently, we provide three types of UniversalTM One-step Cell-based ELISA kit: Kit-R, Kit-M and Kit-G. The Kit-R is suitable for using together with rabbit primary antibodies, the Kit-M is suitable for using together with mouse primary antibodies, and the Kit-G is suitable for using together with goat primary antibodies. Each kit is sufficient to perform 1000 assays in 96-well plates.
Direct detection of protein expression in cells.
Easy: No plate-coating, no cell-lysat-preparing, and no need of secondary antibody.
Rapid: Get results around one hour.
Universal: Suitable for most primary antibodies and no need of secondary antibody.
Reproducible: Highly reproducible results.
Enhancer: for continuous use, store in 2~8 íŠ for up to 3 month, or store in -20 íŠ for up to a year.
50X ELISA Probe: for continuous use, store in -20 íŠ for up to 6 months or store in -70 íŠ manual defrost freezer for up to a year. Repeated freezing and thawing is not recommended.
Fixatives, Pretreatment Solution and Rapid Wash Solution: store in room temperature for up to one year.
Note: Kit will be shipped in ambient temperature.